A typical 1 bun serving of hamburger bun contains 4.3 grams of protein (9.77 g per 100 g), which makes it a moderate source of protein. That covers roughly 9% of the 50 g reference Daily Value, so it adds toward your protein total for the day.
Around 14% of the calories in hamburger bun come from protein, so it is more of an energy food than a protein food. It contributes to a day's protein total, but the bulk of what it delivers is energy rather than amino acids - treat it as a contributor alongside a denser source, not as the protein in a meal.
Among the 111 grains in the database, hamburger bun ranks 54th by protein per serving, and 523rd out of all 796 foods on the site. Set against buckwheat flour, another grain entry in the database, a serving carries about 0.5 g more protein (buckwheat flour has 3.8 g per 1/4 cup).
It is an incomplete protein on its own; pairing it with other plant foods across the day covers the full range of essential amino acids. The serving above is counted by piece at 44 g. Because pieces vary, the per-100 g figure is the one to use when comparing this against a food measured a different way.
Source: USDA FoodData Central #172796 - "Rolls, hamburger or hotdog, plain" (SR Legacy, published 4/1/2019). Values are the published reference figures for that record, not lab measurements for a specific brand or batch.
Quick answers
Hamburger bun protein FAQ
How much protein is in hamburger bun per 100 g?
Hamburger bun has 9.77 g of protein per 100 g. A typical 1 bun serving (44 g) provides 4.3 g.
Is hamburger bun a complete protein?
No. Hamburger bun is an incomplete protein on its own; pairing it with other protein sources across the day covers the full range of essential amino acids.
How much of the Daily Value does a serving of hamburger bun cover?
A 1 bun serving of hamburger bun contains 4.3 g of protein, about 9% of the 50 g reference Daily Value.
Is hamburger bun a good source of protein?
Hamburger bun is a moderate source of protein, and about 14% of its calories come from protein, which makes it more of an energy food than a protein food. Set against buckwheat flour, another grain entry in the database, a serving carries about 0.5 g more protein (buckwheat flour has 3.8 g per 1/4 cup).