A typical 1/4 cup serving of rye flour contains 2.8 grams of protein (10.9 g per 100 g), which makes it only a minor source of protein. That covers roughly 6% of the 50 g reference Daily Value, so it adds toward your protein total for the day.
Around 12% of the calories in rye flour come from protein, so it is more of an energy food than a protein food. It contributes to a day's protein total, but the bulk of what it delivers is energy rather than amino acids - treat it as a contributor alongside a denser source, not as the protein in a meal.
Among the 111 grains in the database, rye flour ranks 86th by protein per serving, and 602nd out of all 796 foods on the site. Set against oatmeal bread, another grain entry in the database, a serving carries about 0.5 g more protein (oatmeal bread has 2.3 g per 1 slice).
It is an incomplete protein on its own; pairing it with other plant foods across the day covers the full range of essential amino acids. The serving above is measured by volume at 25.5 g. The per-100 g figure lets you compare it directly against any other food here, whatever its serving.
Source: USDA FoodData Central #168886 - "Rye flour, medium" (SR Legacy, published 4/1/2019). Values are the published reference figures for that record, not lab measurements for a specific brand or batch.
Quick answers
Rye flour protein FAQ
How much protein is in rye flour per 100 g?
Rye flour has 10.9 g of protein per 100 g. A typical 1/4 cup serving (25.5 g) provides 2.8 g.
Is rye flour a complete protein?
No. Rye flour is an incomplete protein on its own; pairing it with other protein sources across the day covers the full range of essential amino acids.
How much of the Daily Value does a serving of rye flour cover?
A 1/4 cup serving of rye flour contains 2.8 g of protein, about 6% of the 50 g reference Daily Value.
Is rye flour a good source of protein?
Rye flour is only a minor source of protein, and about 12% of its calories come from protein, which makes it more of an energy food than a protein food. Set against oatmeal bread, another grain entry in the database, a serving carries about 0.5 g more protein (oatmeal bread has 2.3 g per 1 slice).