A typical 1/4 cup serving of barley flour contains 3.9 grams of protein (10.5 g per 100 g), which makes it a moderate source of protein. That covers roughly 8% of the 50 g reference Daily Value, so it adds toward your protein total for the day.
Around 12% of the calories in barley flour come from protein, so it is more of an energy food than a protein food. It contributes to a day's protein total, but the bulk of what it delivers is energy rather than amino acids - treat it as a contributor alongside a denser source, not as the protein in a meal.
Among the 111 grains in the database, barley flour ranks 64th by protein per serving, and 550th out of all 796 foods on the site. Set against melba toast, another grain entry in the database, a serving carries about 0.5 g more protein (melba toast has 3.4 g per 1 oz).
It is an incomplete protein on its own; pairing it with other plant foods across the day covers the full range of essential amino acids. The serving above is measured by volume at 37 g. The per-100 g figure lets you compare it directly against any other food here, whatever its serving.
Source: USDA FoodData Central #169739 - "Barley flour or meal" (SR Legacy, published 4/1/2019). Values are the published reference figures for that record, not lab measurements for a specific brand or batch.
Quick answers
Barley flour protein FAQ
How much protein is in barley flour per 100 g?
Barley flour has 10.5 g of protein per 100 g. A typical 1/4 cup serving (37 g) provides 3.9 g.
Is barley flour a complete protein?
No. Barley flour is an incomplete protein on its own; pairing it with other protein sources across the day covers the full range of essential amino acids.
How much of the Daily Value does a serving of barley flour cover?
A 1/4 cup serving of barley flour contains 3.9 g of protein, about 8% of the 50 g reference Daily Value.
Is barley flour a good source of protein?
Barley flour is a moderate source of protein, and about 12% of its calories come from protein, which makes it more of an energy food than a protein food. Set against melba toast, another grain entry in the database, a serving carries about 0.5 g more protein (melba toast has 3.4 g per 1 oz).