A typical 1 cup cooked serving of shiitake mushrooms contains 2.3 grams of protein (1.56 g per 100 g), which makes it only a minor source of protein. That covers roughly 5% of the 50 g reference Daily Value, so it adds toward your protein total for the day.
Only about 11% of the calories in shiitake mushrooms come from protein, so it is more of an energy food than a protein food. It is worth eating for other reasons; the protein it adds to a day is incidental.
Among the 105 vegetables in the database, shiitake mushrooms ranks 41st by protein per serving, and 633rd out of all 796 foods on the site. Set against kimchi, another vegetable entry in the database, a serving carries about 0.6 g more protein (kimchi has 1.7 g per 1 cup).
It is an incomplete protein on its own; pairing it with other plant foods across the day covers the full range of essential amino acids. The serving above is measured by volume at 145 g. The per-100 g figure lets you compare it directly against any other food here, whatever its serving.
Source: USDA FoodData Central #168437 - "Mushrooms, shiitake, cooked, without salt" (SR Legacy, published 4/1/2019). Values are the published reference figures for that record, not lab measurements for a specific brand or batch.
Quick answers
Shiitake mushrooms protein FAQ
How much protein is in shiitake mushrooms per 100 g?
Shiitake mushrooms has 1.56 g of protein per 100 g. A typical 1 cup cooked serving (145 g) provides 2.3 g.
Is shiitake mushrooms a complete protein?
No. Shiitake mushrooms is an incomplete protein on its own; pairing it with other protein sources across the day covers the full range of essential amino acids.
How much of the Daily Value does a serving of shiitake mushrooms cover?
A 1 cup cooked serving of shiitake mushrooms contains 2.3 g of protein, about 5% of the 50 g reference Daily Value.
Is shiitake mushrooms a good source of protein?
Shiitake mushrooms is only a minor source of protein, and about 11% of its calories come from protein, which makes it more of an energy food than a protein food. Set against kimchi, another vegetable entry in the database, a serving carries about 0.6 g more protein (kimchi has 1.7 g per 1 cup).